Kiryl D. Piatkevich, Ph.D.

School of Life Sciences

Molecular BioEngineering Group

CONTACT

Email: kiryl.piatkevich@westlake.edu.cn

Website: www.piatkevich-lab.com

Kiryl D. Piatkevich, Ph.D.

School of Life Sciences

Molecular BioEngineering Group

CONTACT

Email: kiryl.piatkevich@westlake.edu.cn

Website: www.piatkevich-lab.com

"Westlake University offers a world-class research environment where scientists have the freedom and opportunity to unleash their creativity for solving society’s most pressing issues. As the first private school in China, Westlake University will also provide a new paradigm in teaching and mentoring to cultivate the next generation of scientific leaders. Here, every student, every teacher has a unique opportunity to create and shape the future of Westlake University."


Biography

Kiryl  Piatkevich is an Assistant Professor in the School of Life Sciences at Westlake  University. Since early childhood, Dr. Piatkevich was fond of chemistry and  pursuing his passion for science he enrolled in Lyceum of Belarussian State  University at age of 15 to study chemistry. Following the graduation from the  Lyceum, Dr. Piatkevich continued his education at Lomonosov Moscow State  University while doing research in metalorganic, analytical, physical, and  bio-organic chemistry. After receiving a Master of Science degree in Chemistry  with honors, Dr. Piatkevich was accepted to a joint Ph.D. program between Moscow  State University and Albert Einstein College of Medicine in New York to work on  the development of the innovative approaches for intravital imaging of small  mammals. After receiving a Ph.D. in Chemistry, Dr. Piatkevich joined  Massachusetts Institute of Technology as a Postdoctoral Associate to do research  in the Synthetic Neurobiology group led by Dr. Edward Boyden, a world-recognized  pioneer in optogenetics and neuro-engineering. During postdoctoral training, he  worked on novel methods of neural interfacing using synthetic biology and  developed several new molecular technologies for observing neuronal activity in  live behaving animals. Dr. Piatkevich was the first to perform the optical  recording of neuronal activity with high temporal precision in major  neuroscience model organism, including worms, fish, and mice. Starting in 2019,  Dr. Piatkevich leads the Molecular Bioengineering Group in the School of Life  Sciences (www.piatkevich-lab.com).

 

Research

Dr. Piatkevich  research is focused on invention and engineering  of new cutting-edge molecular and imaging technologies for analyzing,  controlling, and repairing complex biological systems such as the brain.  Engineering the genetically encoded molecular tools for optical recording and  manipulation of neuronal activity, as well as fluorescent probes for the  anatomical tracing of neurons is an interdisciplinary research program, which  will span synthetic and chemical biology, bioengineering, biochemistry,  biotechnology, molecular in vivo imaging, neuroscience, robotics, and cell  biology. His group applies developed technologies systematically to reveal  underpinning molecular mechanism of brain disorder and to understand ground  truth principles of neural codes. The new molecular technologies are also  utilized to create advanced brain machine interfaces by means of  light.

 

Representative  Publications

1. Piatkevich  K.D.#, Bensussen S.#, Tseng H.#, Shroff S.N., Lopez-Huerta V.G., Park D., Jung  E.E., Shemesh O.A., Straub C., Gritton H.J., Romano M.F, Costa E., Sabatini  B.L., Fu Z, Boyden E.S., Han X. Population imaging of neural activity in awake  behaving mice. Nature 2019, in  press (# equal  contribution).

2. Qian Y.#,  Piatkevich K.D.#, McLarney B.#, Abdelfattah A.S., Mehta S., Gottschalk S.,  Molina R.S., Zhang W., Drobizhev M., Hughes T.E., Zhang J., Schreiter E.R.,  Shoham S., Razansky D., Boyden E.S., Campbell R.E. A Genetically Encoded  Near-Infrared Fluorescent Calcium Ion Indicator. Nature Methods, 16 (2), 171 (#  equal contribution).

3. Piatkevich  K.D.#, Jung E.E.#, Straub C., Linghu C., Park D., Suk H.J., Hochbaum D.R.,  Goodwin D., Pnevmatikakis E., Pak N., Kawashima T., Yang C.-T., Rhoades J.L.,  Shemesh O., Asano S., Yoon Y.-G., Freifeld L., Saulnier J., Riegler C., Engert  F., Hughes T., Drobizhev M., Szabo B., Ahrens M.B., Flavell S.W., Sabatini B.L.,  Boyden E.S. A Robotic multidimensional directed evolution approach applied to  fluorescent voltage reporters. Nature Chemical Biology, 14 (4), 352 (# equal  contribution).

4. Piatkevich  K.D., Suk H.J., Suhasa B.K., Yoshida F., DeGennaro E.M., Dro bizhev M., Hughes  T.E., Desimone R., Boyden E.S.*, Verkhusha V.V. Near-infrared fluorescence  proteins engineered from bacterial phytochromes in neuroimaging. Biophys. J.  113(10), 2299-2309.

5. Piatkevich  K.D., English B.P., Malashkevich V.N., Xiao H., Almo S.C., Singer R.H.,  Verkhusha V.V. Photoswitchable red fluorescent protein with a large Stokes  shift. Chem Biol. 2014. 21:1402.

6. Piatkevich  K.D.*, Murdock M.H., Subach F.V.* Advances in engineering and application of  optogenetic indicators for neuroscience. Applied Sciences. 2019, 9(3), 562.  (*co-corresponding).

7. Tillberg  P.W.#, Chen F.#, Piatkevich K.D., Zhao Y., Yu C.C., English B.P., Gao L.,  Martorell A., Suk H.J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G.,  Seneviratne U., Tannenbaum S.R., Desimone R., Cai D., Boyden E.S.  Protein-retention expansion microscopy of cells and tissues labeled using  standard fluorescent proteins and antibodies. Nature Biotechnology. 34 (9),  987-992 (# equal contribution).

8. Shemesh  O.A., Tanese D., Zampini V., Linghu C., Piatkevich K., Ronzitti E.,  Papagiakoumou E., Boyden E.S., Emiliani V. Temporally precise single-cell  resolution optogenetics. Nature Neuroscience. 20(12), 1796-1806.

9. Subach O.M,  Barykina N.V., Anokhin K.V., Piatkevich K.D., Subach F.V. Near-Infrared  Genetically Encoded Positive Calcium Indicator Based on GAF-FP Bacterial  Phytochrome. Int. J. Mol. Sci., 20(14), 3488.

10. Subach  O.M., Kunitsyna T.A., Mineyeva O.A., Lazutkin A.A., Bezryadnov D.V., Barykina  N.V., Piatkevich K.D., Ermakova Y.G., Bilan D.S., Belousov V.V., Anokhin K.V.,  Enikolopov G.N.*, Subach F.V.* Slowly reducible genetically encoded green  fluorescent indicator for in vivo and ex vivo visualization of hydrogen  peroxide. Int. J. Mol. Sci. 20(13), 3138;

11. Barykina  N.V., Doronin D.A., Subach O.M., Sotskov V.P., Plusnin V.V., Ivleva O.A.,  Gruzdeva A.M., Kunitsyna T.A., Ivashkina O.I., Lazutkin A.A., Malyshev A.Y.,  Smirnov I.V., Varizhuk A.M., Pozmogova G.E., Piatkevich K.D., Anokhin K.V.,  Enikolopov G.N., Subach F.V*. NTnC-like genetically encoded calcium indicator  with a positive and enhanced response and fast kinetics. Scientific Reports, 8  (1), 15233.

12. Doronin D.,  Barykina N., Subach O., Isaakova E., Varizhuk A., Pozmogova G., Piatkevich K.,  Anokhin K., Enikolopov G., Subach F.* Genetically encoded calcium indicator with  NTnC-like design and enhanced fluorescence contrast. BMC Biotechnology, 18 (1),  10.

13. Barykina  N.V., Subach O.M., Piatkevich K.D., Jung E.E., Malyshev A.Y., Smirnov I.V.,  Bogorodskiy A.O., Borshchevskiy V.I., Varizhuk A.M., Pozmogova G.E., Boyden  E.S., Anokhin K.V., Enikolopov G.N., Subach F.V.*. Green fluorescent genetically  encoded calcium indicator based on calmodulin/M13-peptide from fungi. PLoS One.  12(8):e0183757.

14. Rowlands  C.J., Park D., Bruns O.T., Piatkevich K.D., Fukumura D., Jain R.K., Bawendi  M.G., Boyden E.S., So P.T.C.*. Wide-field three-photon excitation in biological  samples. Light: Science & Applications. 6, e16255.

15. Barykina  N.V., Subach O.M., Doronin D.A., Sotskov V.P., Roshchina M.A., Kunitsyna T.A.,  Malyshev A.Y., Smirnov I.V., Azieva A.M., Sokolov I.S., Piatkevich K.D., Burtsev  M.S., Varizhuk A.M., Pozmogova G.E., Anokhin K.V., Subach F.V.*, Enikolopov  G.N.*. A new design for a green calcium indicator with a smaller size and a  reduced number of calcium-binding sites. Scientific Reports.  6:34447.

16. Konold  P.E., Yoon E., Lee J., Allen S.L., Chapagain P.P., Gerstman B.S., Regmi C.K.,  Piatkevich K.D., Verkhusha V.V., Joo T., Jimenez R.*. Fluorescence from multiple  chromophore hydrogen-bonding states in the far-red protein TagRFP675. J. Phys.  Chem. Lett. 7, 3046-3051.